In the first investigation, a MFand the CPP penetratin were found to increase m NP delivery to cells grown in3D.
In the second investigation, Au NPs were assessed in a range of different celltypes (grown in 2D) for their performance in 4 main areas; cellular toxicity,cellular uptake, c-myc knockdown and effect on the cell cycle.
Nanotechnology has brought a large number of engineered nanomaterials and nanoparticles to applications in multiple daily products and in almost every sector of society.
The many advantages that this relatively new science has brought to our daily life are evident, but still little is known on the potential environmental and human risk posed by nanotechnology applications.
In this thesis, I have studied and engineered the interface of Au NPs with different biological systems, demonstrating a large variety of biomedical applications by modulation of these interfaces.
My research was initially focused on systematically tuning the physicochemical properties of nanoparticles to understand nano-bio interactions at the cellular level.
The main problem facing the development of these novel therapies isthe specific delivery of nucleic acids into diseased cells within the body.
It ishoped that nanoparticles (NPs) can be used to overcome this problem, by actingas vehicles to transport nucleic acids through the body for specific delivery intodiseased cells.
This thesis investigates the removal and removal mechanisms of engineered nanomaterials and nanoparticles in simulated drinking water treatment plants.
It focuses on how engineered nanomaterials and nanoparticles affect process performances and which treatments are best to follow for their removal.